Ataxin-3 Fluorescent Assay Kit

Catalog #

78044

$765 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Ataxin-3 Fluorescent Assay Kit is designed to measure Ataxin-3 activity for screening and profiling applications, in a homogeneous assay with no timeconsuming washing steps.

Need us to run inhibitor screens or profile your compounds against Ataxin-3? Check out our Protease Screening Services or Ubiquitination Screening Services.

This product has been cited 3 times.

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Synonyms

ATX3 kit, ATXN3 kit, JOS kit, MJD1 assay kit

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Product Info

Storage and Usage

Citations3

Assay Kit Format

Fluorogenic

Supplied As

The kit comes in a convenient 96-well format, with purified Ataxin-3 protein, Ubiquitin-AMC, and Ataxin-3 assay buffer for 100 enzyme reactions.

Format

Catalog Number	Component	Amount	Storage
80399	Ataxin-3	500 µg	-80°C	Avoid freeze/ thaw cycles!
81150	Ub-AMC (10 μM)	200 µl	-80°C
78045	Ataxin-3 Assay Buffer	5 ml	-20°C
79685	Black, low binding black microtiter plate	1	Room Temp

UniProt #

P54252

Background

Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS) or Machado-Joseph disease protein 1 (MJD1), is implicated in Machado-Joseph disease, a neurological disease that progresses cerebellar ataxia. It is also clinically relevant in gastric, lung, and testicular cancers.

References

1. Shi, Z., et al. 2018. Ataxin-3 promotes testicular cancer cell proliferation by inhibiting anti-oncogene PTEN. Biochem Biophys Res Commun. 503(1):391-396. doi:10.1016/j.bbrc.2018.06.047
2. Koch, P., et al. 2011. Excitation-induced ataxin-3 aggregation in neurons from patients with Machado–Joseph disease. Nature 480(7378): 543-546.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Deubiquitinase

Regulatory Status

Research Use Only