Anti–SIRT1 monoclonal antibody

Catalog #

25316

$335 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Monoclonal antibody raised in mouse against human SIRT1 (NAD–dependent deacetylase sirtuin–1) using a synthetic peptide

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Synonyms

hSIR2, SIR2alpha, SIR2L1

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Product Info

Storage and Usage

Citations

Isotype

IgG1

Clonality

monoclonal

Host Species/Expression System

Mouse

Purification

Protein G purified

Format

Aqueous buffer solution

Formulation

PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

50 µg/72 µl

Immunogen

synthetic peptide

Background

SirT1 (UniProtKB/Swiss–Prot entry Q96EB6) is a NAD–dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by the deacetylation of key regulatory proteins. Deacetylation of lysine 382 of p53/TP53 by Sirt1 impairs its ability to induce the proapoptotic program and modulate cell senescence, whereas deacetylation of TAF1B represses rDNA transcription by the RNA polymerase I. SirT1 is also involved in HES1– and HEY2–mediated transcriptional repression and inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. SirT1 is recruited to the nuclear bodies via its interaction with PML

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Western blot analysis using the monoclonal antibody directed against hSIRT1
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the monoclonal antibody against hSIRT1 (cat. #25316) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.

Applications

WB (1:500)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Human. Other species: not tested

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–SIRT1 monoclonal antibody

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