ADPRS, His-Tag Recombinant

Catalog #

101852

$350 *

Size: 25 µg

Qty

Bulk/Custom

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Description

Recombinant human ADPRS (ADP-ribosyl-acceptor hydrolase 3, or ARH3), full length encompassing amino acids 2-363(end). This construct contains an N-terminal His-tag (6xHis). This protein was affinity purified.

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Synonyms

ADP-ribosylhydrolase ARH3, ADP-ribose glycohydrolase ARH3, ADP-ribosylhydrolase 3, O-acetyl-ADP-ribose deacetylase ARH3, Poly(ADP-ribose) glycohydrolase ARH3, ADPRHL2, ARH3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

ADPRS Activity

ARH3 inhibition curve using a fluorogenic assay (#82158). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

ADPRS (His-2-363(end))

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT

40 kDa

Amino Acids

2-363(end)

Genbank #

NM_017825.3

UniProt #

Q9NX46

Tag(s)

N-terminal His-Tag

Background

ADPRS, also known as ADP-ribosyl-acceptor hydrolase 3 or ARH3, is part of the DNA damage response machinery, and removes ADP-ribose from serine residues in a Mg²⁺-dependent manner. It acts sequentially to PARG (poly(ADP-ribose) glycohydrolase) and it has a protective role by decreasing the levels of PAR in the cell, which stops mitochondria from releasing PAR-driven AIF (apoptosis inducing factor). Mutations that result in loss of function of this protein lead to CONDSIAS (stress-induced childhood-onset neurodegeneration with variable ataxia and seizures), a disease with multiple clinical expressions. The development and use of ADPRS inhibitors allows us a better understanding of the DNA damage response pathway and opens new avenues for cancer treatment.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Various concentrations of ARH3 were incubated at room temperature with 4 µM TFMU-ADPr (substrate) and the fluorogenic product was measured after 1 hour (λexcitation: 385 nm / λemission: 502 nm).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

DNA Replication and Repair

Regulatory Status

Research Use Only