WRN Helicase Activity Assay Kit
The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN Helicase Activity Assay Kit comes in a convenient 96-well format, with contains enough purified recombinant WRN, ATP, DNA substrate, assay buffer and additives for 100 reactions.
Figure 1: Illustration of the assay principle.
The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence
Need us to run inhibitor screens or profile your compounds against WRN? Check out our DNA Replication & Repair Screening Services.
Fluorescent microplate reader capable of reading λexc/λem=525 nm/592 nm.
| Catalog # | Name | Amount | Storage |
| 101264 | WRN (517-1093), GST-Tag* | 10 µg | -80°C |
| 4x WRN Buffer | 4 ml | -20°C | |
| DNA Substrate | 20 µl | -80°C | |
| ATP (200 mM) | 50 µl | -20°C | |
| 0.5 M DTT | 200 µl | -20°C | |
| 79685 | Black, low binding plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
The WRN gene was first discovered as the gene mutated in Werner syndrome, a recessive genetic disorder characterized by segmental progeria and elevated cancer predisposition. WRN is a multifunctional enzyme with helicase and exonuclease activities and plays roles in various cellular processes crucial for the maintenance of genome stability, including DNA replication, transcription, DNA repair, and telomere maintenance. WRN depletion causes cell cycle arrest, DNA damage, mitotic defects, chromosome shattering, and apoptosis. Loss of heterozygosity involving the WRN loci at chromosome 8p11.2-p12 occurs frequently in many different cancers, pointing to its role as a tumor suppressor gene. Small molecule inhibitors of WRN can be used to induce synthetic lethality and offer a new therapeutical approach for cancer treatment.
Mendoza, O., et al. 2015. Nucleic Acids Res 43(11): e71.
Van Wietmarschen, N., et al. 2021. Curr Opin Genet Dev 71: 34-38.
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