UbcH6 (UBE2E1), His-tag Recombinant

Catalog #

80316

$250 *

Size: 100 µg

Qty

Bulk/Custom

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Description

UbcH6, also known as UBE2E1 (GenBank Accession No. NM_003341), a.a. 2-end, with N-terminal His-tag, MW = 22 kDa, expressed in an E. coli cell expression system.

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Synonyms

UbcH6, UBE2E1

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Product Data Gallery

UbcH6 inhibition curve using a TR-FRET assay (#78862). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

22 kDa

Amino Acids

2–end

Genbank #

NM_003341

UniProt #

P51965

Tag(s)

N-terminal His-tag

References

1. Sommer, T., et al. 1993. Nature 365 (6442):176-9.
2. Yang, M. et al. 1997. J. Biol. Chem. 272 : 1970-1975.

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 µl reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH6, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, then Europium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin Conjugating Enzyme (E2)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80316_Lot91119.pdf

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