Trypsin-2 Activity Assay Kit
The Trypsin-2 Activity Assay Kit is a colorimetric assay kit designed to measure trypsin-2 protease activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant trypsin-2, substrate, and assay buffer for 100 enzyme reactions.
Figure 1: Illustration of the assay principle.
Upon proteolysis, trypsin-2 cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at λ=405 nm. The increase in color is proportional to trypsin-2 activity.
- Microplate reader capable of reading at λ=405 nm
- Adjustable micropipettor and sterile tips
| Catalog # | Name | Amount | Storage |
| Human Trypsin-2 (1 µg/µL) | 10 µg | -80°C | |
| PR Substrate 3 | 1 ml | -80°C | |
| PR-02 Buffer | 10 ml | -20°C | |
| 79963 | 96-well clear microplate | 1 | Room Temp |
Trypsin is a serine protease from the pancreas, that hydrolyzes peptides specifically at the carboxyl side of arginine and lysine residues. It is formed as a proenzyme (trypsinogen) in the pancreas and secreted to the duodenum when the pancreas is activated by cholecystokinin. It is then converted to its active form in the digestive system by enterokinase, where it supports digestion by degrading proteins into smaller peptides. Trypsin has multiple applications in biotechnology, being commonly used in tissue culture to resuspend adherent cells, dissociate cells from tissues, generate peptides for proteomic studies and in multiple food related processes. Early activation of trypsin in the pancreas can result in pancreatitis. Trypsin is also involved in SARS-CoV2 (severe acute respiratory syndrome coronavirus 2), by enabling viral entry. Trypsin inhibitors are present in several foods, such as soybeans, as a mechanism of defense against digestion, and protect animals from self-digesting the pancreas. TATI (peptide-tumor-associated trypsin inhibitor) is a marker of late stage ovarian, gastric and pancreatic cancer and it is found at high levels in kidney failure patients. The use of trypsin inhibitors as antibacterial and anti-viral agents in the food industry and as therapy has been under investigation and may offer a promising approach as bactericides.
Kim Y., et al., 2022 Arch Virol. 167(2): 441-458.
Nascimento A., et al., 2022 J Enzyme Inhib Med Chem 37(1):749-759.
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