TAK1-TAB1 Kinase Assay Kit
The TAK1-TAB1 Kinase Assay Kit is designed to measure TAK1-TAB1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in-vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog # | Reagent | Amount | Storage | |
| 40279 | TAK1-TAB1, GST-tag* | 5 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer | 1.5 ml | -20°C | |
| 79686 | ATP (500 μM) | 100 µl | -20°C | |
| 78514 | MBP (5 mg/ml) | 50 µl | -20°C | |
| 79696 | 96-well plate, white | 1 | Room Temp |
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*The concentration of TAK1-TAB1 is lot-specific and will be indicated on the tube containing the enzyme
TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (NF-κB). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. It has been implicated in various inflammation disorders and cancers, including breast and pancreatic cancer.
1. Zhu, J., et al. 2015. "Expression of TAK1/TAB1 expression in non-small cell lung carcinoma and adjacent normal tissues and their clinical significance." International Journal of Clinical and Experimental Pathology 8(12): 15801-15807.
2. Sakurai, H. 2012. "Targeting of TAK1 in inflammatory disorders and cancer." Trends in Pharmacological Sciences 33(10): 522-530
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