TAF1L (BD2), GST-tag Recombinant

Catalog #

31106

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, also known as TAF1L, GenBank Accession No. NM_153809, a.a. 1517 – 1649 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 42.3 kDa, expressed in an E. coli expression system.

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Synonyms

TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, TAF1L

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Product Data Gallery

TAF1L (1517-1649) a-screen

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol.

42.3 kDa

Amino Acids

1517-1649

Genbank #

NM_153809

UniProt #

Q8IZX4

Tag(s)

N-terminal GST-tag

References

Wang, PJ., et al., Hum. Mol. Genet. 2002 Sep 15; 11(19): 2341-2346.

Storage/Stability

>6 months at –80°C.

Assay Conditions

TAF1L (1517-1649) protein is incubated with biotinylated acetyl- histone peptide substrate (Cat. #33000) in TAF1L assay buffer in a 10 µl reaction for 1 hour at RT. AlphaLISA® GSH acceptor beads (PerkinElmer) are added, and the mixture is incubated for 30 minutes at room temperature (RT). AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer) are then added. After 10 minute incubation at RT, measure Alphacounts.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only