STK3(MST2), GST-tag Recombinant

Catalog #

40013

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human STK3 (serine/threonine kinase 3), also known as MST-2 (Mammalian STE20-like protein kinase 2) or KRS1, GenBank Accession No. NM_006281, a.a. 2-492(end), with N-terminal GST-tag, MW=78.4 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

KRS1; MST2; FLJ90748

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥41%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol

78.4 kDa

Amino Acids

2–492(end)

Specific Activity

31 pmole/min/µg

Genbank #

NM_006281

UniProt #

Q13188

Tag(s)

N-terminal GST-tag

Background

STK3, also known as MST2, encodes a protein of 491-amino acid which contains an N-terminal catalytic domain characteristic of STKs. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall. RAF1 has been shown to counteract apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2). STK3 is a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand. STK3 activation presumably allows cells to resist unfavorable environmental conditions.

References

1. Taylor, L.K., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(19):10099-104.
2. Song, H., et al. (2010). Proc. Natl. Acad. Sci. USA. 107(4):1431-6.
3. Kim, D., et al. (2010). PLoS One 5(3):e9616.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Ser/Thr Peptide 7, Invitrogen) at 30°C for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40013_Lot101012.pdf

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STK3(MST2), GST-tag Recombinant

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