SLK, GST-tag Recombinant

Catalog #

40275

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human SLK, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

LOSK, STK2, se20-9, KIAA0204, MGC133067, bA16H23.1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥80%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

180 kDa

Amino Acids

full length

Genbank #

AB002804

UniProt #

Q9H2G2

Tag(s)

N-terminal GST-tag

Background

SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex (1). Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

References

1. Burakov A V, et al: LOSK (SLK) protein kinase activity is necessary for microtubule organization in the interphase cell centrosome. Dokl Biol Sci. 2005;403:317-9.
2. Chaar Z, et al: v-Src-dependent down-regulation of the Ste20-like kinase SLK by casein kinase II. J Biol Chem. 2006 Sep 22;281(38):28193-9.

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SLK, GST-tag Recombinant

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