PKG1β, His-tag Recombinant

Catalog #

40083

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PKG1β (beta) (GenBank Accession No. NM_006258), full length with N-terminal His-tag, MW = 78 kDa, expressed in Baculovirus infected Sf9 cell expression system.

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Synonyms

CGK1B, PRKG1B, cGMP-dependent, type I, cGKIb

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Product Data Gallery

PKG1beta Specific Activity

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.

78 kDa

Amino Acids

full length

Specific Activity

0.85 pmoles/min/µg

Genbank #

NM_006258

UniProt #

Q13976

Tag(s)

N-terminal His-tag

References

1. Bivalacqua T.J. et al., BJU Int. 2007 Jun,99(6):1488-94.
2. Lee J.H. et al., Retrovirology. 2007 Dec 13,4:91.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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PKG1β, His-tag Recombinant

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