PDE5A1 (Mouse), GST-tag Recombinant

Catalog #

60051

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE5A, phosphodiesterase 5A, PDE5-A1, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PDE5A1 (Mouse) inhibition curves using a fluorescence polarization assay (#79602). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, 10% glycerol.

124 kDa

Amino Acids

full length

Specific Activity

≥ 4630 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.

Genbank #

NM_153422

UniProt #

Q8CG03

Tag(s)

N-terminal GST-tag

References

1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 0.1 mg/ml BSA, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of mPDE5A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only