PADI1 Recombinant

Catalog #

50151

$320 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human PADI-1, also known as Peptidyl arginine deiminase, type I, (GenBank Accession No. NM_013358), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PADI-1, Peptidyl arginine deiminase, type I, Pad

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.

75 kDa

Amino Acids

1-663(end)

Specific Activity

≥35 pmol/min/μg

Genbank #

NM_013358

UniProt #

Q9ULC6

Tag(s)

C-terminal FLAG-tag

References

1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813–23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384–93.
3. Guerrin M, et al. (2003). Biochem. J. 370 (Pt 1): 167–74.

Storage/Stability

>6 months at –70°C.

Assay Conditions

Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing PADI1 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.
After 30 min incubation at 37°C, the reaction was quenched using 15 µL of 500 mM EDTA (final concentration is 115 mM) followed by measuring the amount of NH3 produced by o-phthalaldehyde (OPT) and dithiothreitol (DTT). Fluorescence was measured at λ_ex413 nm and λ_em476 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles

Scientific Category

Arginine Deiminase

Regulatory Status

Research Use Only