OX40/NF-κB Luciferase Reporter HEK293 Cell Line

Catalog #

60482

$10,650 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The OX40/NF-κB Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing the Firefly luciferase reporter under the control of NF-κB (nuclear factor kappa light chain enhancer of activated B cells)-responsive elements and OX-40 (Tumor Necrosis Factor Receptor Superfamily Member 4, GenBank Accession No. NM_003327).

This cell line has been validated by flow cytometry, and with OX40L and anti-OX40 antagonist antibodies.

Figure 1: Illustration of mechanism of action of the OX40/NF-κB Luciferase Reporter HEK293 Cell Line.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of OX40 without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb, nfkb, nf-kb

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Product Data Gallery

Cell surface expression of OX40 in OX40/NF-κB Luciferase Reporter HEK293 Cell Line.

Dose curve response of OX40/NF-κB Luciferase Reporter HEK293 Cell Line to OX40L

Dose curve response of OX40/NF-κB Luciferase Reporter HEK293 Cell Line to Anti-OX40 Antagonist Antibody, in the presence of OX40L.

Product Info

Storage and Usage

Citations2

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1A	BPS Bioscience #79528

Materials Used in Cellular Assay

Name	Ordering Information
Assay Medium: Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1A	BPS Bioscience #79528
OX40L (CD252), His-tag (Human) Recombinant	BPS Bioscience #71185
Anti-OX40 Antagonist Antibody	BPS Bioscience #72063
Clear-bottom, white 96-well tissue culture-treated plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

UniProt #

P43489

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

OX40, also known as CD134, is a co-stimulatory receptor, of the TNF (tumor necrosis factor) receptor family, expressed on the surface of T cells. Binding of OX40 to its ligand, OX40L (also known as CD252), potentiates T cell activation, differentiation, proliferation, survival, and T cell effector function. OX40L is present in NK cells, participating in their activation and cytotoxicity profile, and dendritic cells. OX40 can bind to members of the TRAF (TNFR associated factor) family of proteins, which can then regulate the NF-κF (nuclear factor kappa-light chain enhancer of activated B cells) signaling pathway. OX40 and OX40L can be found in cancer cells, such as AML (acute myeloid leukemia) and breast cancer cells. Studies have shown that OX40 agonists can increase anti-tumor immunity and improve tumor-free survival in pre-clinical studies. Alternatively, OX40 antagonists offer potential as therapeutics for inflammatory diseases. The development of new modulators of the OX40/OX40L activity are promising therapies for patients suffering from solid tumors or auto-immune disorders.

References

Peng K., et al., 2014 AAPS J. 16(4): 625–633.
Marconato M., et al., 2022 Scientific Reports 12: 15856.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1A (BPS Bioscience #79528): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B and 400 µg/ml G418.

Media Required for Functional Cellular Assay
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Applications

Characterize OX40 activity and binding to ligands.
Screen for compounds that regulate OX40 signaling in a cellular model.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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OX40/NF-κB Luciferase Reporter HEK293 Cell Line

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