NKp46 Lentivirus (Macaca fascicularis/Cynomolgus)

Catalog #

78779

$850 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NKp46 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with Macaca fascicularis (also known as crab-eating macaque or cynomolgus monkey) NKp46 (Q95JB9.1) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection gene (Figure 1).

Figure 1: Schematic of the lenti-vector used to generate the cynomolgus NKp46 Lentivirus.

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Synonyms

CD335, Natural Cytotoxicity Triggering Receptor 1, Lymphocyte Antigen 94 Homolog, and Natural Killer Cell P46-Related Protein

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Product Data Gallery

Expression of NKp46 CHO-K1 cells using cynomolgus NKp46 lentivirus.

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.  

Background

NKp46 (also known as Natural Cytotoxicity Triggering Receptor 1, NCR1, and CD335) is an important NK-activating receptor expressed on the surface of human NK (natural killer) cells, involved in cellular cytotoxicity. NKp46 participates in the activation of NK cells against pathogens, tumor cells, and virally infected cells; it also plays an important role in autoimmune conditions. NKp46 expression level can be used as a prognostic tool in acute myeloid leukemia (AML). Due to its crucial role in NK cell activity NKp46 has been used in multiple therapeutical approaches, such as trifunctional NK cell engagers (combined with CD16 and a cancer target) and strategies aimed at increasing NKp46 expression to generate higher NK cell-mediated cytotoxicity.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Expression of NKp46 in cells of interest.
Generate stable cell lines expressing cynomolgus NKp46 (puromycin resistant).

Shipping Temperature

-80°C

Notes

To generate a NKp46 stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin (as pre-determined from a killing curve), for an antibiotic selection of transduced cells. Visit: https://bpsbioscience.com/cell-line-faq for guidelines on performing a kill curve.

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For further questions, email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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NKp46 Lentivirus (Macaca fascicularis/Cynomolgus)

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