NKp46 CHO Cell Line (High, Medium, or Low Expression)

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Catalog #

78915

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Description

NKp46 CHO Cell Line is a clonal CHO cell line stably expressing full-length human NKp46 (Natural cytotoxicity triggering receptor 1) (NM_004829). Surface expression of NKp46 was confirmed by flow cytometry. Stable clonal cell lines were selected for different levels of NKp46 expression (low, medium, and high) compared to the parental CHO-K1 cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Cell surface expression of NKp46 in NKp46 CHO Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains ≥1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3J	BPS Bioscience #79974

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

NKp46 (also known as Natural cytotoxicity triggering receptor 1, or NCR1) is an important natural killer (NK) cell-activating receptor expressed on the surface of human NK cells, and it is involved in cytotoxicity. NKp46 participates in the activation of NK cells against pathogens, tumor cells, and virally infected cells. It also plays an important role in autoimmune conditions, including type I diabetes. NKp46 expression is often conserved on infiltrating NK cells in most solid tumors, and NKp46 is a diagnostic biomarker and possible therapeutic target for gastrointestinal T-cell lymphoproliferative diseases. Although a few infectious NKp46 ligands have been identified, endogenous NKp46 cell surface ligands are unknown. Recently, it was reported that NKp46 binds to externalized calreticulin (ecto-CRT), which translocate from the ER (endoplasmic reticulum) to the plasma membrane in situations of cellular stress such as chemotherapy and senescence. This leads to NK cell degranulation and target cell killing and can participate in controlling cancer. Further studies will elucidate the exact mechanism and roles of NKp46 and its ligands in disease and allow the development of new therapeutics for cancer therapy.

References

Sivori S., et al., 1999 Eur J Immunol. 29 (5):1656-66.
Gardner G. and Fraker C.A., 2021 Front Immunol. 12:722979.
Sen Santara S., et al., 2023 Nature 616 (7956):348-356.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3J (BPS Bioscience #79974): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 5 µg/ml of Puromycin.

Applications

Screen and validate antibodies against NKp46 for drug discovery and research.
Perform binding assays to screen for potential NKp46 ligands.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only