Mouse Vascular Endothelial Growth Factor 120AA Recombinant

Catalog #

90253-B

$205 *

Size: 10 µg

Qty

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Description

Recombinant Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of 121 amino acid residues, and migrates as an approximately 28 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Mouse Vascular Endothelial Growth Factor mature chain was expressed in E. coli.

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Synonyms

VEGF, Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGF-120, VEGF121, VEGF120

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

E. coli

Purity

≥96% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered solution of 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.

28 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

27–146

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be in the range of 2.0-4.0 ng/ml.

Genbank #

Q00731

UniProt #

Q00731

Background

VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 , 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. Kaposi sarcomas express VEGF121 and VEGF165. VEGF121 and VEGF165 are soluble secreted forms of the factor while VEGF189 and VEGF206 are mostly bound to heparin-containing proteoglycans in the cell surface or in the basement membrane. A high-affinity glycoprotein receptor of 170-235 kDa is expressed on vascular endothelial cells. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. The high-affinity receptor for VEGF, now known as VEGFR1, has been identified as the gene product of the FLT-1. Another receptor for VEGF, now known as VEGFR2, is KDR, also known as FLK-1. A third receptor type, VEGFR3 is known also as FLT- 4. An isoform-specific receptor for VEGF165 has been identified as human Neuropilin-1.

References

1. Kawai H, et al. Lung Cancer. 2008 Jan,59(1):41-7.
2. Pan Q, et al. J Biol Chem. 2007 Aug 17,282(33):24049-56.

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Mouse Vascular Endothelial Growth Factor 120AA Recombinant

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