Mouse PKM2, His-Tag Recombinant

Catalog #

50296

$505 *

Size: 20 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Murine Pyruvate kinase M2 (PKM2), also known as PKM1/M2, isoform a (GenBank Accession No. NM_011099), a.a. 2-end, with N-terminal His-tag, MW = 58 kDa, expressed in an E. coli expression system.

This product has been cited 1 time.

●

Synonyms

pyruvate kinase muscle, PKM2, cytosolic thyroid hormone-binding protein,CTHBP, thyroid hormone-binding protein 1, THBP1, opa-interacting protein 3,OIP3

●

Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations1

Species

Mouse

Host Species/Expression System

E. coli

Purity

>90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

58 kDa

Amino Acids

2–end

Specific Activity

≥11 pmol/min/µg.

Genbank #

NM_011099

UniProt #

P52480

Tag(s)

N-terminal His-tag

Background

PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.

References

1. Spoden G.A. et al., Exp. Cell Res. 315 (16), 2765-2774 (2009).
2. Christofk H.R. et al., Nature 452 (7184), 230-233 (2008).

Storage/Stability

>6 months at –80°C

Assay Conditions

A 50 µl PKM2 reaction is conducted in a buffer containing 50 mM Tris (pH 7.4), 100 mM MgCl₂, 250 mM KCl, 40 mM ADP, 100 mM phosphoenolpyruvate (PEP) at room temperature for 15 min. ATP production is detected using Kinase-Glo Luminescent Kinase Assay Platform.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes, Nucleotide Kinase

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

50296_Lot91013.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

Mouse PKM2, His-Tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY