KMO, His-FLAG-Tags Recombinant

Only %1 left

Catalog #

11307

As low as $145 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human KMO (kynurenine 3-monooxygenase), full length, encompassing amino acids 1-486(end). This construct contains a C-terminal His-Flag-tag (6xHis). The recombinant protein was affinity purified.

●

Synonyms

Kynurenine 3-Monooxygenase, Kynurenine 3-Hydroxylase, KMO, and EC 1.14.13.9, kmo

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

60%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 μg/ml FLAG peptide, 0.2 mM TCEP and 20% glycerol.

58 kDa

Amino Acids

1-486 (end)

Specific Activity

100 pmol/min/μg

Genbank #

NM_003679

UniProt #

O15229

Tag(s)

C-terminal His-FLAG-tags

References

1. Zwilling, D., et. al., Cell. 2011; 6(10):863-874.
2. Giorgini, G., et al., Nature Genetics. 2005; 37: 526-531.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

KMO #11307 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 μM NADPH, 400 μM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm were measured using a Tecan Infinite M1000 plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only