Recombinant human IDH2 (human isocitrate dehydrogenase 2), full length encompassing amino acids 1-452(end). This construct contains a C-terminal FLAG-tag. This protein contains mutation of interest (R140Q). The recombinant protein was affinity purified.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and variable FLAG peptide.
MW
52 kDa
Amino Acids
1–452(end)
Specific Activity
≥61 pmol/min/µg
Genbank #
NM_002168
UniProt #
P48735
Tag(s)
C-terminal FLAG-tag
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). The IDH2 homodimer is an the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production and may tightly associate or interact with the pyruvate dehydrogenase complex.
References
1. Schaap, F.G., et al., Adv. Anat. Pathol. 2013;20(1):32-38. 2. Grassian, A.R., et al., J. Biol. Chem. 2012;287(50):42180-42194.
Assay run according to IDH2(R140Q) Assay Kit (BPS Bioscience #79309) with IDH2 titrated at various concentrations.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.