IDH2 (R140Q), FLAG-Tag Recombinant

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Catalog #

71100

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Description

Recombinant human IDH2 (human isocitrate dehydrogenase 2), full length encompassing amino acids 1-452(end). This construct contains a C-terminal FLAG-tag. This protein contains mutation of interest (R140Q). The recombinant protein was affinity purified.

This product has been cited 2 times.

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Synonyms

Isocitrate Dehydrogenase 2, IDH2, IDH-2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

IDH2 Activity

IDH2(R140Q) inhibition curve using a fluorogenic assay (#79309). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

IDH2 (R140Q) (1-452(end)-FLAG)

Mutation

R140Q

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and variable FLAG peptide.

52 kDa

Amino Acids

1–452(end)

Specific Activity

≥61 pmol/min/µg

Genbank #

NM_002168

UniProt #

P48735

Tag(s)

C-terminal FLAG-tag

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). The IDH2 homodimer is an the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production and may tightly associate or interact with the pyruvate dehydrogenase complex.

References

1. Schaap, F.G., et al., Adv. Anat. Pathol. 2013;20(1):32-38.
2. Grassian, A.R., et al., J. Biol. Chem. 2012;287(50):42180-42194.

Application Reference(s):
1. Identification of a selective inhibitor of IDH2/R140Q enzyme that induces cellular differentiation in leukemia cells (2020)