Human Monocyte Chemotactic Protein-1 Recombinant

Catalog #

90211-A

$130 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human MCP-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant MCP-1 is a disulfide-linked monomeric protein consisting of 77 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human MCP-1 (CCL2) mature chain was expressed in E. coli.

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Synonyms

MCP-1, CCL2, MCAF, Monocyte Chemotactic Protein, HC11, Chemokine (C-C Motif) Ligand 2, MCP1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥98% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.4.

9 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

24–99

Biological Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

Genbank #

P13500

UniProt #

P13500

Background

MCP-1 (Monocyte Chemotactic Protein-1) belongs to the family of chemotactic cytokines. MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells. MCP-1 has been shown to exhibit biological activities other than Chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK. Elevated levels of MCP-1 are observed in atherosclerotic plaques that are rich in macrophages. The factor activates the tumoricidal activity of monocytes and macrophages in vivo. It regulates the expression of cell surface antigens (CD11c, CD11b) and the expression of cytokines (IL-1, IL-6). MCP-1 is a potent activator of human basophils, inducing degranulation and the release of histamines. In basophils activated by IL-3, IL-5 or GM-CSF, MCP-1 enhances the synthesis of leukotriene C4.

References

1. . Biol. Chem., Oct 2009, 284: 27620 - 27628.
2. Am J Physiol Heart Circ Physiol, Oct 2009, 297: H1208 - H1216.
3. J. Immunol., Sep 2009, 183: 3463 - 3471.

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Human Monocyte Chemotactic Protein-1 Recombinant

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