Human Interleukin-7 Recombinant

Catalog #

90198-B

$205 *

Size: 10 µg

Qty

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Description

Recombinant human IL-7 is a disulfide-linked monomeric protein consisting of 153 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in E. coli.

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Synonyms

IL-7, Interleukin 7, IL7

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 4.5

17 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

26–177

Biological Activity

The ED₅₀ determined by dose-dependent stimulation of the proliferation of murine 2E8 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.

Genbank #

P13232

UniProt #

P13232

Background

IL-7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL-7. Human (152 amino acids) and murine IL-7 (129 amino acids) show 60% sequence homology at the protein level. The human IL-7 receptor, designated CD127, is an integral strongly glycosylated membrane protein of 76 kDa expressed on activated T-cells. IL-7 receptors are expressed on pre-B-cells and their progenitors and bone marrow macrophages, but they are not expressed on mature B-cells. Functional IL-7 receptors are also found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B-cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line.

References

1. J. Exp. Med., Sep 2009, 206: 2111 - 2119.
2. J. Immunol., Sep 2009, 183: 3130 - 3138.
3. Blood, Aug 2009, 114: 1768 - 1775.

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Human Interleukin-7 Recombinant

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