TDO-HEK293 Recombinant Cell line
Catalog #
60534
$10,340
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Purchase
Description
Stable recombinant HEK293 cell line expressing human tryptophan 2,3 dioxygenase (TDO2, TDO, TPH2, TRPO), Genbank accession number NM_005651.1.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
tryptophan 2,3 dioxygenase, TDO2, TDO, TPH2, TRPO
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Product Data Gallery
Product Info
Storage and Usage
Citations
Host Cell Line
HEK293
Materials Required But Not Supplied
• Assay Medium: Thaw Medium 1 (BPS Cat. #60187)
• Growth Medium 1B (BPS Cat. #79531)
• Geneticin
• PBS
• 0.05% Trypsin EDTA
• 6.1 N Trichloroacetic acid (Sigma #T0699)
• Acetic acid (Sigma #320099)
• TDO Cellular Activity QuickDetectTM Supplements (BPS Cat. #62002). Note: other formulations can be used, but significant optimization may be required.
UniProt #
P48775
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit
(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in
mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key
regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an
immunosuppressive microenvironment by starving immune cells of L-Trp and releasing
degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3
dioxygenase (TDO), is upregulated in many tumors, providing cancer cells with an avenue for
immune evasion.
References
1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.