Firefly Luciferase THP-1 Cell Line

Catalog #

78409

$1,900 *

Size: 2 vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant THP-1 cells constitutively expressing the firefly (Photinus pyralis) luciferase gene under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Product Data Gallery

Luciferase activity in the Firefly Luciferase THP-1 Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, Human Monocytic Leukemia, suspension

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2R	BPS Bioscience #78411

Materials Required for Cellular Assay

Name	Ordering Information
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

THP-1 is a human monocytic leukemia cell line, derived from the peripheral blood of a patient with acute monocytic leukemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. The THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation and exploring the regulation of macrophage-specific genes. The use of a reporter such as luciferase allows for an easy ready out in several assays, with the signal generated by the firefly luciferase being proportional to the cell number.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience, #60184): RPMI-1640 medium supplemented with 10% FBS (Fetal bovine Serum), 1% Penicillin/Streptomycin.

Growth Medium 2R (BPS Bioscience, #78411): RPMI-1640 medium supplemented with 10% FBS (Fetal bovine Serum), 1% Penicillin/Streptomycin, plus 400 μg/ml Hygromycin B.

Applications

Use as an internal control in CAR-T co-culture killing assays.
In vitro and in vivo bioluminescence imaging.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only