Firefly Luciferase SKOV-3 Cell Line

Catalog #

78425

$1,900 *

Size: 2 vials

Qty

Bulk/Custom

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Description

Recombinant SKOV-3 cells constitutively expressing the firefly (Photinus pyralis) luciferase reporter gene under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in Firefly Luciferase SKOV-3 recombinant cells

Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Background

SKOV-3 is a human cell line with epithelial-like morphology that was derived from the ascitic fluid of a patient with ovarian adenocarcinoma. Dysfunctional apoptosome activation, a characteristic of SKOV-3, is linked to the pathogenesis of ovarian carcinoma and chemoresistance. This cell line is hypodiploid and exhibits high tumorigenicity when injected into nude mice. It is also resistant to tumor necrosis factors and some cytotoxic drugs. SKOV-3 is an ideal model for research on estrogen and antiestrogen-resistant ovarian cancer. The signal generated by the firefly luciferase is proportional to cell numbers.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 7 (BPS Bioscience #60185): McCoy’s 5A medium with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 7D (BPS Bioscience #78200): McCoy’s 5A medium with 10% FBS, 1% Penicillin/Streptomycin, plus 100 μg/ml of Hygromycin

Applications

Use as an internal control in CAR-T or NK co-culture killing assays
In vitro and in vivo bioluminescence imaging

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only