Firefly Luciferase KG1 Cell Line

Catalog #

78493

$1,900 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Firefly Luciferase KG1 Cell Line is a KG-1 cell line constitutively expressing the firefly (Photinus pyralis) luciferase reporter under the control of a CMV promoter.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity in Firefly Luciferase KG-1 Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

KG-1, human myeloblast cell line derived from the bone marrow of a patient with acute myelogenous leukemia.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 15	BPS Bioscience #78412
Growth Medium 15A	BPS Bioscience #78413

Materials Required for Cellular Assay

Name	Ordering Information
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-Well Flat Bottom Microplate, TC-Treated	Corning #3610
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

KG-1 is a human myeloblast cell line derived from the bone marrow of a patient with acute myelogenous leukemia. KG-1 cells possess the unique feature of being completely dependent on colony-stimulating factors (CSFs) for colony formation and have myeloid characteristics. Further, KG-1 has been used as a model system to study dendritic cell differentiation. The cell line KG-1 is an ideal model system for the study of myeloid differentiation and cancer therapies. The signal generated by the firefly luciferase is proportional to the cell number and provides an easy assay readout.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 15 (BPS Bioscience #78412): IMDM medium supplemented with 20% FBS, 1% Penicillin/Streptomycin.

Growth Medium 15A (BPS Bioscience #78413): IMDM medium supplemented with 20% FBS, 1% Penicillin/Streptomycin, plus 100 µg/ml Hygromycin B.

Applications

Use as an internal control in CAR-T or NK co-culture killing assays
In vitro and in vivo bioluminescence imaging

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only