DAT – CHOK1 Recombinant Cell Line

Catalog #

60558

$9,400 *

Size: 2 vials

Qty

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Description

Recombinant CHOK1 cell line expressing Human sodium-dependent dopamine transporter (DAT), Genbank accession number NM_001044.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

SLC6A3, sodium-dependent dopamine transporter

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Product Data Gallery

Western Blot of hDAT expression in DAT-CHO-K1 cells

Dose response of hDAT activity in DAT-CHO-K1 cells to reference inhibitor Amfebutamone HCl. The IC50 of Amfebutamone HCl is ~ 268 nM

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Supplied As

Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

• Neurotransmitter Transporter Uptake Assay Kit (Molecular Devices #R6138)
• HBSS buffer (1×) (Hyclone #SH30588.01).
• Thaw Medium 3 (BPS Bioscience #60186)
• Growth Medium 3B (BPS Bioscience #79529)

UniProt #

Q01959

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

Background

The dopamine transporter (DAT, SLC6A3) is a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synaptic cleft back into the cytosol, from which other transporters sequester DA and norepinephrine (NE) into vesicles for storage and later release. Dopamine reuptake via DAT provides the primary mechanism through which dopamine is cleared from synapses. DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, and alcoholism.

References

1. Vaughan, RA., et al., Trends in Pharmacological Sciences. 2013; 34(9): 489–496.
2. Jorgensen, S., et al., Journal of Neuroscience methods. 2008; 169: 168-176.

Storage/Stability

Immediately upon receipt, store in liquid nitrogen.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Cat. #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Bioscience #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B to ensure the recombinant expression is maintained.

Instructions for Use

See data sheet for detailed culturing and assay protocol

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Membrane transporter

Regulatory Status

Research Use Only