CSL Reporter – HEK293 Cell line

Catalog #

79754

$2,275 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Notch CSL Reporter – HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) stably integrated into HEK293 cells. Transfection of this cell line with a Notch expression vector and activation of the Notch pathway leads to expression of luciferase reporter. This cell line is validated with a Notch1DelE expression vector, which is constitutively processed by γ-secretase, leading to the release of NICD and the expression of luciferase.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of Notch signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Notch reporter, PKA reporter, cAMP reporter, luciferase reporter , CSL reporter, CBF1 reporter, RBP-J reporter, Cell based assay kit, cell-based assay, reporter assay kit

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Product Data Gallery

DAPT Blocked Notch Reporter Activity

Product Info

Storage and Usage

Citations1

Supplied As

Each vial contains ~1.5 X 106 cells in 1 ml of FBS with 10% DMSO.

Materials Required But Not Supplied

• Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)
• Growth Medium 1B (BPS Bioscience #79531)
• Lipofectamine 2000 (Invitrogen #11668027) or other transfection reagent
• DAPT (Selleckchem #S2215): inhibitor of Notch pathway (γ-secretase inhibitor). Prepare 10 mM of stock solution in DMSO
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate (Corning #3610)
• ONE-Step™ Luciferase Assay System (BPS Bioscience #60690)
• Luminometer

Background

The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues. Notch signaling is triggered through the binding of a transmembrane ligand to Notch transmembrane receptor (NOTCH1/ NOTCH2/NOTCH3/NOTCH4) on a neighboring cell. This results in proteolytic cleavage of the Notch receptor, releasing the constitutively active intracellular domain of NOTCH (NICD). NICD translocates to the nucleus and associates with transcription factors CSL (CBF1/RBPJκ/Suppressor of Hairless/Lag-1) and coactivator Mastermind to turn on transcription of Notch-responsive genes.

References

Lu, F.M., et al. (1996) Constitutively active human Notch1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element. Proc. Natl. Acad. Sci. USA 93(11): 5663-5667.

Kanungo, J., et al (2008) The Notch signaling inhibitor DAPT down-regulates cdk5 activity and modulates the distribution of neuronal cytoskeletal proteins. J. Neurochem. 106: 2236-2248.

Storage/Stability

Immediately upon receipt, store cells in liquid nitrogen.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 μg/ml Geneticin®

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only