Chemi-Verse™ RSK4 Kinase Assay Kit

Catalog #

82523

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ RSK4 Kinase Assay Kit is designed to measure RSK4 (ribosomal S6 kinase 4) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough recombinant purified RSK4, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against RSK4? Check out our Kinase Screening and Profiling Service.

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Synonyms

Ribosomal protein S6 kinase alpha-6, S6K-alpha-6, 90 kDa ribosomal protein S6 kinase 6, p90-RSK 6, p90RSK6, Ribosomal S6 kinase 4, RSK4, RSK-4, pp90RSK4

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Product Data Gallery

Inhibition of RSK4 kinase activity by RSK4-IN-1.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
	RSK4*	2 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	RSK Substrate (5 mg/ml)	100 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q9UK32

Background

RSK4 (ribosomal S6 kinase 4) is a member of the serine/threonine RSK family of proteins. In vertebrates this family has four isoforms, RSK1-4, involved in cell growth, proliferation, motility, and apoptosis, acting as downstream effectors of the Ras/ERK (extracellular signal regulated kinase) pathway. RSK4 is found at high levels in the kidney, the brain, and the thyroid gland. It is linked to ESCC (esophageal squamous cell carcinoma) where it is found at high levels and phosphorylates GSK-3β to activate the β-catenin signaling pathway and leads to resistance to radiotherapy. Inhibition of this protein with the inhibitor BI-D1870 was able to increase the sensitivity of the ESCC cells to radiotherapy in animal models. In glioblastoma RSK4 phosphorylates EZH2 (enhancer of zeste homolog 2) and contributes to resistance to treatment with EZH2 inhibitors. RSK4 contributes to treatment resistance and cancer progression in kidney carcinoma, melanoma and RCC (renal cell carcinoma) cell lines, but the role of RSK4 as a tumor suppressor or oncogene may be cancer dependent and remains controversial. A deeper understanding of the role of this protein in cancer combined with the use of inhibitors alone or in combinatory therapy may prove a new therapeutic avenue in cancer therapy.

References

Li M.-Y., et al., 2020 J Clin Invest 130 (8) : 4301-4319.
Pang F., et al., 2023 Cancer Gene Ther. 30(12) :1636-1648.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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Chemi-Verse™ RSK4 Kinase Assay Kit

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