CDK12/Cyclin K Assay Service
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Target
CDK12/Cyclin K
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Description
Screening and/or profiling inhibitor compounds against CDK12/Cyclin K kinase activity in a biochemical assay.
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Synonyms
CRK7, Cdc2-Related Kinase, KIAA0904, CRKRS, CRKR, Cell Division Cycle 2-Related Protein Kinase, Cell Division Protein Kinase 12, CDC2-Related Protein Kinase 7, Cyclin-Dependent Kinase 12, CDC2 Related Protein Kinase 7, HCDK12, CrkRS
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
THZ531, 0.2 μM
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Assay Principle
The CDK12/ Cyclin K Kinase Assay is designed to measure the activity of complex CDK12/ Cyclin K for screening and profiling applications using luminescent Kinase-Glo® MAX as a detection reagent.
Target Details
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Protein Family
DNA Replication and Repair, Kinases
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UniProt
CDK12: Q9NYV4; CyclinK: O75909
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Background
The CDK12/Cyclin K complex comprises human CDK12 (Cyclin Dependent Kinase 12) and human Cyclin K. Cyclin-dependent kinase (CDK) complexes have been implicated in the regulation of transcription. The CDK12/ Cyclin K complex regulates phosphorylation of Serine 2 in the C-terminal domain of RNA polymerase II, which is responsible for productive transcriptional elongation and synthesis of full-length mature mRNAs. This complex contributes to the maintenance of genome stability and plays a role in embryonic development as well as cancer.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
