CD73 Inhibitor Screening Assay Kit
The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity for screening and profiling applications. The CD73 assay kit comes in a convenient 384-well format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric detection reagent for 384 enzyme reactions.
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 630 nm
Adjustable micropipettor and sterile tips
37˚C incubator Rotating or rocker platform (optional)
Aluminum foil
Catalog Number |
Component |
Amount |
Storage |
|
71184 | CD73 | 5 µg | -80°C | Avoid freeze /thaw cycles! |
74000 | 5x CD73 assay buffer | 3 x 1 ml | -20°C | |
79496 | AMP (500 μM) | 2 x 1 ml | -20°C | |
74001 | Colorimetric detection reagent* | 2 x 10 ml | +4°C | |
79962 | Transparent 384-well plate | 1 | Room Temp. |
*Colorimetric detection reagent is used to measure the free phosphate from the CD73 reaction. Any source of inorganic phosphate can interfere with the assay.
CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activity or decreasing CD39 expression.
Antonioli, L., et al., Trends in Cancer, Vol. 2, No. 2, 95-109.
Application Reference(s):
1. Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes (2017)