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Custom Lentivirus Services BPS Bioscience has developed a diverse portfolio of lentivirus products to study COVID-19 , cell signaling pathways , CAR T-cell therapy , CRISPR , and immunotherapy . In addition to our product portfolio , we also offer custom services to develop a lentivirus suited for your research needs.  Customizable Options Reporters ( GFP , Luciferase , Dual Reporter ) Antibiotic selection markers ( Puromycin , Hygromycin , G4...

CRISPR Products & Services Cells Lentiviruses Proteins Services CRISPR-Cas9 is a tool used for genome editing that has been adapted from the bacteria’s innate immune response. Bacteria capture and store DNA fragments from invading viruses or DNA within a region of their genome termed the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) array. This region is continually transcribed, producing short CRISPR RNA (cr...

Gene & Cell Therapy Gene and cell therapies are paving the way for innovative treatments that can offer new hope for patients with previously untreatable diseases. Gene therapy and cell therapy are cutting-edge medical approaches aimed at revolutionizing healthcare. Gene therapy involves the manipulation and introduction of genetic material into a patient's cells to correct genetic disorders or enhance therapeutic effects. This can be achieved by ...

The Power of Lentiviruses in Cell Engineering Introduction The ability to manipulate nucleic acids has been a key discovery that has driven research, and ultimately clinical applications, forward. It opened the door to study the impact of mutations in cellular models, dissect pathways, generate recombinant proteins and monoclonal antibodies, and is the basis of gene and cell therapy, continuing to lead to new discoveries and cure patients every day. The methods used to ...

Viral Tools Frequently Asked Questions FAQ Topic: -Any- General Lentiviruses AAVs FAQ Search: How should I store the viral particles (lentivirus, AAV or VSV) that I just rec...

Frequently Asked Questions BPS Bioscience is here to answer your questions and provide project guidance. On the following pages, we outline some of the most frequently asked questions separated by product type. If your question is not answered through these resources, please contact us today and we are happy to assist.  Proteins Cell Lines Assay Kits ...

AAV Tools Graham Beards’ Own work CC BY-SA 4.0 Adeno-Associated Virus (AAV) is a small dependoparvovirus which was initially discovered as a contaminant in adenovirus preparations. AAVs are non-enveloped and consist of an icosahedral capsid containing a short, single-stranded DNA genome flanked by two Inverted Terminal Repeat sequences (ITRs). AAVs are replication-defective, non-pathogenic, and h...

CRISPR/Cas9 Kinase Knockout Lentivirus Panel Each CRISPR/Cas9 Kinase Knockout Lentivirus is designed to target a specific kinase of interest for knockout. The replication-incompetent, HIV-based, VSV-G pseudotyped lentiviral particles are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The SIN (self-activation) lentiviral backbone contains the Cas9 gene ( Streptococcus pyogenes CRISPR associated protein 9) driven by an EF1a promoter, an sgRNA dr...

Lentiviruses Lentiviral transduction is a powerful tool for introducing genes into cells and is used extensively for cellular engineering. It has recently made its way to the clinic with ex-vivo cell therapy applications. Lentiviruses (LV) have also been used extensively for the modeling of SARS-CoV-2 viral infection of human cells. Lentiviral transduction is a powerful tool for introducing genes into cells and is used extensively for cellular engineering...

Structure and Function of the Claudin Family Introduction Claudins (encoded by the multi-gene family CLDN) are tetraspan membrane proteins found in all epithelial and endothelial cells. They serve as crucial structural components of tight junction strands, where the membranes of two adjacent cells connect to form a barrier by self-polymerization and transcellular interactions at the apical-lateral membrane of epithelia. The tight junctions prevent molecules from passing bet...

CRISPR-Based Synergistic Activation Mediator (SAM) Introduction Cas9 ( Streptococcus pyogenes CRISPR-associated protein 9) is a sequence-specific prokaryotic endonuclease that is recruited to the DNA by a single guide RNA (sgRNA) and introduces a double stranded break into the DNA. This double stranded break is usually repaired through Non-Homologous End Joining (NHEJ) in mammalian cells (1) . The target DNA sequence requires only 23 bp (base pairs) in length and sho...

Cell Lysis Buffers Selecting the right cell lysis buffer is a critical step in many research applications across various scientific disciplines, ranging from molecular biology to cell biology and biochemistry. The choice of cell lysis buffer can significantly impact the quality and reliability of experimental results, making it a fundamental consideration for researchers. This importance stems from several key factors. &n...

Citations   This page contains a list of scientific journal articles that have cited BPS products and services since 2013. We would like to congratulate all our loyal customers on their contributions to the scientific community. Our customers are truly amazing and dedicated scientists and this is evidenced by the outstanding research they are doing.  Search By Product Name or Catalog Number Name Title 10X HAT Assay Buffer (50095...