Native Nucleosome (HEK293-derived)

Catalog #

52015

$695 *

Size: 0.6 mg

Qty

Bulk/Custom

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Description

Nucleosomes prepared from mammalian HEK293 cells as a substrate for histone methyltransferase assays.

This product has been cited 5 times.

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations5

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 3 mM DTT, 10 mM Na butyrate, and 20% glycerol

Background

Nucleosomes are portions of DNA that are wrapped around histone proteins in the form of a histone octamer. The histone octamer consists of two copies of H2A, H2B, H3 and H4. Nucleosomes correspond to subunits of chromatin and allow the DNA to be compacted enough to fit into the cell’s nucleus. The presence of nucleosomes at specific points of the genome determines the ability of regulatory proteins to access DNA.

References

1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.

Storage/Stability

At least 6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

The concentration of this product is determined by Bradford and represents only the protein concentration.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase/Substrate

Regulatory Status

Research Use Only