20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 3 mM DTT, 10 mM Na butyrate, and 20% glycerol
Background
Nucleosomes are portions of DNA that are wrapped around histone proteins in the form of a histone octamer. The histone octamer consists of two copies of H2A, H2B, H3 and H4. Nucleosomes correspond to subunits of chromatin and allow the DNA to be compacted enough to fit into the cell’s nucleus. The presence of nucleosomes at specific points of the genome determines the ability of regulatory proteins to access DNA.
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful as substrate for histone methyltransferase assays.
Shipping Temperature
-80°C
Notes
The concentration of this product is determined by Bradford and represents only the protein concentration.