BRD2 (BD1+BD2), GST-tag Recombinant

Catalog #

31024

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 – 459 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 71.1 kDa, expressed in an E. coli expression system.

This product has been cited 2 times.

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Synonyms

BRD2, RING, RNF3, Bromodomain containing 2, BRD-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD2-BD1 BD2

BRD2 (BD1+BD2) inhibition curve using a TR-FRET assay (#32614). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 10% glycerol, and 1 mM DTT

71.1 kDa

Amino Acids

65-459

Genbank #

NM_005104

UniProt #

P25440

Tag(s)

N-terminal GST-tag

References

1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.
2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.

Storage/Stability

>6 months at –80°C.

Assay Conditions

BRD2 is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen^® Streptavidin- conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31024_Lot120223G.pdf

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BRD2 (BD1+BD2), GST-tag Recombinant

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