BirA, His-FLAG-tags (E. coli) (E. coli-derived) Recombinant
Catalog #
70030
$540
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Description
Recombinant (e. coli) BirA (Bifunctional ligase/repressor BirA), full length, encompassing amino acids 2-321(end). This construct contains an N-terminal His tag (6xHis) followed by a Flag-tag and a PreScission Protease. This recombinant protein was affinity purified.
This product has been cited 2 times.
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Synonyms
Holocarboxylase Synthetase, Biotin Protein Ligase, ATP-Hydrolysing Ligase
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Product Info
Storage and Usage
Citations2
Species
E. coli
Construct
BirA (E. coli) (His-FLAG-2-321(end))
Host Species/Expression System
E. coli
Purity
≥90%
Format
Aqueous buffer solution
Formulation
50 mM Tris-HCl, pH 8.0, 50 mM NaCl, variable imidazole, 3 mM DTT, and 5% glycerol
MW
37 kDa
Amino Acids
2-321(end)
Genbank #
NP_418404
UniProt #
P06709
References
1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.
2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.