Anti-HLA-DR Biotin-Labeled, Antibody

Catalog #

101769

$300 *

Size: 100 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant human anti-HLA-DR antibody recognizing human HLA-DR (human leukocyte antigen-DR isotype). This anti-HLA-DR antibody is a purified recombinant antibody, which is labeled with biotin using Avi-Tag™ technology at the C-terminus of the heavy chain unit.

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Synonyms

HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, HLA-DRA, HLA-DRA1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Anti-HLA-DR Binding Activity

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 53 kDa + glycans; Light Chain: 23 kDa

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

P01903

Label

This antibody is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

HLA-DR (human leukocyte antigen -DR isotype) is an MHC (major histocompatibility complex) class II protein and it serves as a ligand for the TCR (T cell receptor) when combined with an immunogenic peptide. Matching HLA isotypes is crucial in organ transplant, with HLA-DR being the main HLA isotype responsible for graft-versus host disease during the first 6 months post-transplant. A number of auto-immune diseases are linked to HLA-DR, including Crohn’s disease, lupus, and multiple sclerosis. HLA-DR is found in a subset of NK cells that combine properties from NK and dendritic cells. They are able to produce and release cytokines, degranulate and expand, but also present antigens to CD4⁺ and CD8⁺ T cells. The identification of HLA isotypes and understanding of the mechanisms of action of HLA-DR are thus crucial in medicine.

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

The antibody was validated by ELISA. HLA-DR protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer and washes, the plate was incubated with Streptavidin-HRP (BioLegend #405210), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study the binding of HLA-DR in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Signaling

Regulatory Status

Research Use Only