Anti-GFP-Tag, Monoclonal

Catalog #

25008

$275 *

Size: 100 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Affinity purified Anti-GFP (Green Fluorescent Protein) Tag clone GF28R, mouse IgG2b. Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP. Applications: Western Blot(1:1000-3000), Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Immunoprecipitation (1:50-200). Optimal dilutions/concentrations should be determined by the end user.

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Synonyms

Antibody, GFP-tag

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Product Data Gallery

Product Info

Storage and Usage

Citations

Isotype

IgG2b

Clonality

clone GF28R

Host Species/Expression System

Mouse

Format

Aqueous buffer solution

Formulation

1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN₃

Immunogen

Synthetic peptide conjugated to KLH, corresponding to N-terminal a.a. of GFP from the jellyfish Aequorea victoria.

Background

Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Storage/Stability

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.

Applications

Dot Blot, ELISA, IP, IS, WB

Shipping Temperature

-20°C or-80°C

Species Reactivity

Aequorea victoria.

Cross Reactivity

Recognizes native and denatured forms of jellyfish GFP and its variants such as: EGFP, YFP, EYFP, and CFP. Does not react with coral-derived fluorescent proteins.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Antibodies

Regulatory Status

Research Use Only