ACLY Assay Kit

Catalog #

79904

$620 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. The ACLY Assay Kit is designed to measure ACLY activity for screening and profiling applications using ADP-Glo^® Kinase Assay as a detection reagent. The ACLY Assay Kit comes in a convenient 96-well format, with enough purified recombinant ACLY enzyme, substrate, ATP, and ACLY assay buffer for 100 enzyme reactions.

Need us to run inhibitor screens or profile your compounds against ACLY? Check out our Metabolic Enzyme Screening Services.

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Product Data Gallery

Inhibition of ACLY enzyme

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The ACLY Assay Kit comes in a convenient 96-well format, with enough purified recombinant ACLY enzyme, substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 0.5 M)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Reagent	Amount	Storage
50255	ACLY	3 µg	-80°C	Avoid freeze/ thaw cycles!
	5x ACLY assay buffer	1.5 ml	-80°C
79686	ATP (500 μM)	500 µl	-20°C
	Sodium Citrate (10 mM)	50 µl	-80°C
	Coenzyme A (10 mM)	50 µl	-80°C
79696	96-well plate, white	1	Room Temp

UniProt #

P53396

References

Zaidi, N., J. Swinnen, and K. Smans. 2012. “ATP-Citrate Lyase: A Key Player in Cancer Metabolism.” Cancer Research 72 (15):3709-14.

Storage/Stability

Up to 6 months when stored as recommended.

Assay Conditions

Inhibition of ACLY enzyme by BMS303141, measured using the ACLY Assay Kit, BPS
Bioscience #79904. Data shown is lot-specific. For lot-specific information, please contact
BPS Bioscience, Inc. at [email protected]

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Metabolic Enzyme

Regulatory Status

Research Use Only