ACLY Assay Service
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Target
ACLY
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Description
Screening and/or profiling inhibitor compounds against ACLY lyase activity in a biochemical assay.
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Synonyms
ATP-citrate synthase, citrate cleavage enzyme, ATP-citrate lyase
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Luminescent
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Reference Compounds and IC50
SB 204990, 12 μM
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Assay Principle
The ACLY Assay is designed to measure ACLY activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent that measures ATP formed from ADP using light generated in a luciferase reaction.
Target Details
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Protein Family
Metabolic Enzyme
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UniProt
P53396
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Background
ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. It converts citrate to acetyl-CoA, linking carbohydrate metabolism to fatty acid biosynthesis.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
