USP13, His-Tag, FLAG-Tag Recombinant

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Catalog #

102018

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Description

Recombinant human USP13 (ubiquitin carboxyl-terminal hydrolase 13), encompassing amino acids 2-863(end). This construct contains an N-terminal His-tag (6xHis) and a C-terminal FLAG-tag. This protein was affinity purified.

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Synonyms

Deubiquitinating enzyme 13, Isopeptidase T-3 (ISOT-3), Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, Ubiquitin carboxyl-terminal hydrolase 13, USP-13

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

USP13 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

USP13 (His-2-863(end)-FLAG)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.48 mM KCl, 0.045% Tween-20, 10% glycerol, 100 µg/ml FLAG peptide

100 kDa

Amino Acids

2-863(end)

Specific Activity

5 pmol/min/µg

Genbank #

NM_003940.3

UniProt #

Q92995

Tag(s)

N-terminal His-Tag, C-terminal FLAG-Tag

Background

USP13 (ubiquitin carboxyl-terminal hydrolase 13), also known as isopeptidase T 3 (ISOT-3) is a DUB (deubiquitination) enzyme. It participates in multiple crucial cellular processes, such as autophagy, DNA damage response, ERAD (endoplasmic reticulum-associated degradation) by deubiquitinating proteins involved in those functions. It also plays roles in diseases such as cancer and neurodegeneration. It can play both a suppressor and activator role in tumorigenesis. For instance, low levels of USP13 correlate with low levels of PTEN (phosphatase and tensin homolog), as UPS13 stabilizes the protein. A deeper understanding of all its cellular roles and how to best target its function in a specific context may prove beneficial in cancer therapy.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

USP13 was incubated in 50 mM Tris-HCl, 0.5 mM EDTA, 0.05% Tween 20, 1 mM DTT with 500 nM Ubiquitin-AMC. The reaction was incubated at room temperature for 30 minutes. Fluorescence intensity was measured at λexc/λem of 360nm/460nm using a Tecan M1000 plate reader.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Deubiquitylating Enzymes

Regulatory Status

Research Use Only