SMARCA2 Inhibitor Screening Assay Kit (Discontinued)

Catalog #

32610

$510 *

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Purchase

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Description

The SMARCA2 Inhibitor Screening Assay Kit is designed to measure the inhibition of SMARCA2 binding to its substrate. The key to the SMARCA2 Inhibitor Screening Assay Kit is the specific binding of the SMARCA2 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing SMARCA2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

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Synonyms

SMARCA2, BRM, BRG1-Associated Factor 190B, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin 2, BAF190, SNF2L2, activity assay kit

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

Kit comes in a convenient AlphaLISA^® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified SMARCA2 bromodomain to perform a total of 384 enzyme reactions.

Materials Required But Not Supplied

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA^® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen^® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen^® microplate reader
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
31141	GST-SMARCA2 (1375-1511)	10 μg	-80°C	*Avoid freeze/ thaw cycle*
	Bromodomain Ligand 2	400 μl	-80°C
	Non-acetylated Ligand 2	200 μl	-80°C
33007	3x BRD Homogeneous Assay Buffer 2	4 ml	-20°C
33006	3x BRD Homogeneous Detection Buffer 2	3 ml	-20°C

UniProt #

P51531

References

Filippakopoulos, P., et al., Cell 2012; 149:214.

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.

Shipping Temperature

dry ice

Notes

Contraindications

DMSO above 0.5%. Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the Alphascreen^® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN₃) or metal ions (Fe²⁺, Fe³⁺, Cu²⁺, Zn²⁺ and Ni²⁺). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen^® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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SMARCA2 Inhibitor Screening Assay Kit (Discontinued)

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