PTPσ (PTPRS), GST-tag Recombinant

Catalog #

30045

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human PTPRS (GenBank Accession No. BC104812), a.a. 883-end, with N-terminal GST-tag, MW= 98 kDa, expressed in an E. coli expression system.

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Synonyms

PTPsigma, PTPRS, protein tyrosine phosphatase sigma, PTPσ

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥60%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.

98 kDa

Amino Acids

883–end

Specific Activity

347 pmol/min/µg

Genbank #

BC104812

UniProt #

Q13332

Tag(s)

N-terminal GST-tag

Background

PTPRS is a member of a subfamily of receptor type PTPases that is characterized by the presence of an extracellular cell adhesion molecule-like motif and 2 intracellular phosphatase domains. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis.

References

1. Forrest, A.R. et al., Genome Biol. 2006;7(1):R5.
2. Muise, A. and Rotin, D. Expert Rev Mol. Diagn. 2008 Jul;8(4):465-77.

Storage/Stability

>12 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 2 mM EDTA, 1 mM DTT, 25 mM pNPP. Assay was done at room temperature for 16 minutes, and monitored continuously at Abs405 nM. ε _pNPP = 18,000 M^-1cm^-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Receptor

Regulatory Status

Research Use Only