PTPβ, GST-tag Recombinant

Catalog #

30042

$325 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Protein tyrosine phosphatase PTPβ (beta), also known as PTPRB (GenBank accession No. X54131), a.a. 1675-1996 (catalytic domain) with N-terminal GST-tag, MW=63.7 kDa, expressed in an E. coli expression system.

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Synonyms

PTPB, PTPRB, VEPTP, PTPbeta, RPTbeta, PTP&beta, VE-PTP

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione

63.7 kDa

Amino Acids

1675–1996

Specific Activity

57 nmole/min/µg

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Genbank #

X54131

UniProt #

P23467

Tag(s)

N-terminal GST-tag

Background

Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells.

References

1. I. K. Lund et al., J Biol Chem. 2004, 279: 24226-35.
2. G.H. Peters et al., Biochimie. 2003, 85: 527-34.

Storage/Stability

>12 months at -80°C.

Assay Conditions

20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and PTPβ. Assay was done at 30°C for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε _pNPP= 18,000 M^-1cm^-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

PTP/Receptor

Regulatory Status

Research Use Only