PRMT6 (HRMT1L6) Chemiluminescent Assay Kit

Catalog #
52046
$750 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The PRMT6 Direct Activity Assay kit is designed to measure PRMT6 activity for screening and profiling applications. The key to the PRMT6 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonyms
protein arginine N-methyltransferase 6, PRMT6, Histone-arginine N-methyltransferase, HRMT1L6, PRMT-6
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Species
Human
Supplied As
Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffe
Format
Catalog # Component Amount Storage
51049 PRMT6 human recombinant enzyme 20 µg -80°C  Avoid
freeze/
thaw
cycles
52120 20 µM S-adenosylmethionine*  250 µl -80°C 
52150 Primary antibody 4  100 µl -80°C 
52131H Secondary HRP-labeled antibody 2  10 µl -80°C 
52192 4x HMT assay buffer 6** 3 ml -20°C 
52100 Blocking buffer 4 50 ml +4°C 
  HRP chemiluminescent substrate A (transparent bottle 6 ml +4°C 
  HRP chemiluminescent substrate B (brown bottle) 6 ml +4°C 
  96-well plate precoated with histone substrate  1 plate +4°C 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


*
Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors. 
**Add 31 µl of 0.5 M DTT before use. 

 

UniProt #
Q96LA8
Background
PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3.
References

Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.

Application Reference(s):

1. Peptidic transition state analogues as PRMT inhibitors, (2019)