PLA2G7 (Lp-PLA2), His-tag Recombinant

Catalog #

60091

$450 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human PLA2G7, (GenBank Accession No. NM_005084), a.a. 22-441(end), with C-terminal His-tag, MW=49.2 kDa, expressed in a mammalian cell expression system.

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Synonyms

Phospholipase A2 G7, Lp-PLA2, Lipoprotein-associated phospholipase A2, PAF-AH

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293 cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol.

49.2 kDa

Amino Acids

22-441(end)

Genbank #

NM_005084

UniProt #

Q13093

Tag(s)

C-terminal His-tag

References

1. Berg M., et al., Mol Cancer. 9:100. (2010).
2. Kopec AK., et al., Toxicol Appl Pharmacol.256(2):154-67. (2011).

Storage/Stability

>6 months at –80°C.

Assay Conditions

Enzyme reaction is conducted using liposomally incorporated Red/Green BODIPY PC-A2 (5 µM, Invitrogen) as a substrate in a reaction buffer (50 mM Tris, pH 8.8, 100 mM NaCl, 1 mM CaCl₂). Reaction progress was monitored at ex480/em533 continuously at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Suitable for binding studies, as an immunogen, and as a standard for Western blotting

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Phospholipases

Regulatory Status

Research Use Only