PDGF-BB Recombinant
Catalog #
90800-3
$4,320
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Description
Recombinant PDGF-BB is a disulfide-linked homodimeric protein consisting of a two 109 amino acid residue subunits, and migrates as an approximately 32 kDa protein under non-reducing and 16 kDa under reducing conditions. Optimized DNA sequence encoding human PDGF-BB mature chain was expressed in Saccharomyces cerevisiae cells.
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Synonyms
Platelet-Derived Growth Factor Beta dimer, PDGF-BB, platelet-derived growth factor beta (homodimer), PDGF-Beta
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Saccharomyces cerevisiae
Purity
≥98% by SDS-PAGE and HPLC analysis.
Format
lyophilized protein
Formulation
Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.
Endotoxin Level
Endotoxin level was found to be < 0.1 ng/μg (1EU/μg), using the LAL gel clot method.
Biological Activity
Determined by the dose-dependent proliferation of BALB/c 3T3 cells to be 1.0-3.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 IU/mg.
Genbank #
NM_002608
UniProt #
P01127
References
1. Szabo, A., et al. Stroke 38 (8), 2247-2253 (2007)
2. Guida, T., et al. Clin. Cancer Res. 13 (11), 3363-3369 (2007)
2. Guida, T., et al. Clin. Cancer Res. 13 (11), 3363-3369 (2007)
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