PDE4A Isoform 4, GST-Tag, His-Tag Recombinant

Catalog #

60040

$485 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human full lenght PDE4A (Phosphodiesterase 4A) isoform 4, amino acids 1-647. This construct contains an N-terminal GST-tag, followed by an His-Tag (6xHis) and a thrombin cleavage site. This protein was affinity purified.

This product has been cited 20 times.

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Synonyms

PDE4A isoform 4, phosphodiesterase 4A1, PDE4A1, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE4A1A inhibition curves using a fluorescence polarization assay (#60340). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations20

Species

Human

Construct

PDE4A Isoform 4 (GST-His-Th-1-647(end))

Host Species/Expression System

Sf9 insect cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT

102 kDa

Amino Acids

1-647(end)

Specific Activity

≥9,400 pmol/min/µg

Genbank #

NM_006202.3

UniProt #

P27815

Tag(s)

N-terminal GST-tag

Background

Phosphodiesterases (PDEs) play an important role in the dynamic regulation of the second messengers cAMP (cyclic adenosine monophosphate) and cGMP (cyclic guanosine monophosphate) signaling, by hydrolyzing them. The PDE superfamily is composed of 11 families, with PDE4, 7 and 8 being cAMP-specific hydrolases, and thus regulating positive and negative responses to it. PDE4 is a subfamily of PDEs that includes isoenzymes A to D, and that can be long, short or supershort, to a total of at least 20 different known non-redundant isoforms. PDE4 proteins are involved in cancer, depression, autism, and inflammation. Inhibitors of PDE4 are being used for the treatment of psoriasis, COPD (chronic obstructive pulmonary disease) and atopic dermatitis. PDE4A4 is a long isoform found in several tissues, including the pituitary. It has been linked to GH (growth hormone)-secreting adenomas. It can interact with AIP (AH receptor-interacting protein), a tumor suppressor. The development of PDE4A specific inhibitors and a deeper understanding of the role of each PDE4A isoform in health and disease will prove beneficial.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed with 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, 0.1 mg/ml BSA, and PDE4A4 serial diluted from 200 ng – 0 ng. The reaction was incubated at 37°C for 20 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only