Recombinant HEK293 cell line expressing human PDE10A2 (phosphodiesterase 10A, accession number NM_006661).
N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Format
aqueous solution containing DMSO
Genbank #
NM_006661
UniProt #
Q9Y233
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Background
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE10A plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE10A hydrolyzes both cAMP and cGMP to nucleoside 5?-monophosphate, but exhibits higher affinity for cAMP, and it utilizes cAMP more efficiently as a substrate (Fujishige K. et al., 1999).
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Applications
Useful to monitor cAMP and PDE10A signaling pathway activity and to screen activators or inhibitors of PDE10A.
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.
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