Nectin4 – CHO K1 Recombinant Cell Line (High, Medium, or Low Expression)

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Catalog #

78097

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Description

Recombinant human Nectin4-CHO K1 cell line stably expressing a full length human Nectin4/PVRL4 receptor (GenBank Accession: NM_030916.2). Surface expression of hNectin4 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hNectin4 expression (High, Medium, Low) to mimic different stages of cancer target cells with various Nectin4 expression levels.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Nectin Cell Adhesion Molecule 4 Cell Line, PVRL4 cell line, Nectin-4 cells, Nectin4 cell line

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Product Data Gallery

Flow cytometry analysis of cell surface expression of hNectin4 in CHO K1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO K1 cell line, Chinese Hamster Ovary

Species

Human

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Materials Required But Not Supplied

Materials Required for Cell Culture

Thaw Medium 3 (BPS Bioscience #60186)
Growth Medium 3B (BPS Bioscience #79529)

Genbank #

NM_030916.2

UniProt #

Q96NY8

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

In contrast to other Nectins, which are found extensively in adult tissues, Nectin4 is abundant during fetal development but declines in adult life. Its expression, however, returns specifically in lung, breast, pancreas and ovarian cancers. It was shown that Nectin4 can modulate the expression of epithelial–mesenchymal transition-related proteins via the PI3K/AKT pathway. In addition, the recent study demonstrated that Nectin4 is a cancer-specific TIGIT ligand and its expression is also associated with poor prognostic features, suggesting it could be an efficient target for cancer immunotherapy.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Bioscience, #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only